Plates are incubated anaerobically at 37oC for 3–4 days. Serial dilutions are used for determination of cfu as described above for lactobacilli. However, in the presence of accompanying micro flora, AMC agar (Arroyo et al. 2001) with the aid of the primer pairs which may be genus-specific, as is the case with bifidobacteria. Study 5 Serial Dilution method flashcards from Mandy S. On StudyBlue. Advantages of serial dilution-agar plate; 1. Only viable cells counted 2. Allows isolation of discrete colonies that can be subcultured into pure colonies which can be studied/identified. Disadvantages of serial dilution-agar plate. Overnight incubation required.

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